Available Strain Details

Strain Information
Common Strain Name: E2f3 Pricing and Ordering Information
Strain Nomenclature: B6;129S-E2f3tm1Lees/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B3

Strain Description: Phenotype not fully penetrant on mixed background. Surviving E2F3 null adults die from dilated cardiomyopathy (similar to the human disease) and are smaller than littermates. Embryos dying in utero have defects in heart development. On a pure background all E2F3 null embryos die in utero. Mouse embryo fibroblasts lacking E2F3 have defects in entry into the cell cycle and hence proliferation. Proliferation defects in the embryo are also apparent. E2F3 is a key regulator of proliferation and loss of E2F3 suppresses tumorigenesis in mice heterozygous for a mutation in the tumor suppressor gene pRb.
Mutation Information
Mutation Type: Targeted
Gene Name: E2f transcription factor 3
Gene Symbol: E2f3
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6; 129S
Approx. Generation: 6
Organ Site:
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female).
Genotyping Information: Protocol 1: Allele: E2f3<tm1Lees>
Donating Investigator: Dr. Jacqueline Lees

Key Reference: Humbert P O, Verona R, Trimarchi JM, Rogers C, Dandapani S, and Lees J. 2000 E2f3 is critical for normal cellular proliferation. Genes and Development 14(6):690-703. PMID: 10733529 [Pubmed Abstract]