Available Strain Details

Strain Information
Common Strain Name: Fen1 Pricing and Ordering Information
Strain Nomenclature: B6.Cg-Fen1tm1Rak/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: Absence of Fen1 expression leads to embryonic lethality. Most Fen1 heterozygous animals appear normal however in combination with mutation of the adenomatous polyposis coli (APC) gene double heterozygous animals have increased numbers of adenocarcinomas and decreased survival. Tumors show microsatillite instability.
Mutation Information
Mutation Type: Targeted
Gene Name: Flap endonuclease
Gene Symbol: Fen1
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.Cg
Approx. Generation: 15
Organ Site: GI
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female). The donating investigator suggests maintaining the altered allele by backcrossing animals to C57BL6J rather than by intercrosses of heterozygotes.
Genotyping Information: Protocol 1: Allele: Fen1<tm1Rak>
Donating Investigator: Dr. Raju Kucherlapati

Key Reference: Kucherlapati M, Yang K, Kuraguchi M, Zhao J, Lia M, Heyer, et al. Haploinsufficiency of Flap endonuclease (Fen 1) leads to rapid tumor progression. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9924-9. Epub 2002 Jul 15. PMID: 12119409 [Pubmed Abstract]