Available Strain Details

Strain Information
Common Strain Name: PML (conventional k/o) - C57BL/6 Pricing and Ordering Information
Strain Nomenclature: B6.129S7-Pmltm1Ppp/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B3,C1

Strain Description: The promyelocytic leukemia gene PML was originally identified at the t(15;17) translocation of acute promyelocytic leukemia, which generates the oncogene PML-retinoic acid receptor ?. PML epitomises a subnuclear structure called PML nuclear body. PML controls cell growth and tumorigenesis. Although the incidence of spontaneous tumors in the Pml -/- cohort was not increased during the first year of life and Pml -/- are grossly normal, the role of PML in tumorigenesis and leukemogenesis has been firmly established. The progressive reduction of Pml gene dosage obtained by crossing PML-RARa transgenic mice with Pml -/- mice resulted in a dramatic increase in the incidence of leukemia and in an acceleration of leukemia onset (Rego et al., 2001). In additon, Pml -/- mice are highly susceptible to develop tumors in in-vivo models of chemical-induced carcinogenesis (Wang et al., 1998). Further, analysis of Pml -/- mice also demonstrated that PML exerts its tumor suppressive role at least in part by coordinating a number of proapoptotic responses of critical relevance both in the maintenance of cellular homeostasis in several organs including the bone marrow, as well as in the clearance of cells in response to stress/DNA damage (Wang et al., 1998).
Mutation Information
Mutation Type: Targeted
Gene Name: promyelocytic leukemia
Gene Symbol: PML
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129S7
Approx. Generation: 3
Organ Site:
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is homozygous x homozygous. When maintained as a live colony, breeder pairs were distributed as homozygotes. Average litter size is 8.
Genotyping Information: Protocol 1: Allele: Pml<tm1Ppp>
Donating Investigator: Dr. Pier Paolo Pandolfi

Key Reference: Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7154-9. doi: 10.1073/pnas.1222509110. Epub 2013 Mar 27. Integrated transcriptional and competitive endogenous RNA networks are cross-regulated in permissive molecular environments. Ala U1, Karreth FA, Bosia C, Pagnani A, Taulli R, LĂ•opold V, Tay Y, Provero P, Zecchina R, Pandolfi PP. PMID 23536298 [Pubmed Abstract]

Related Reference: Science. 1998 Mar 6;279(5356):1547-51. Role of PML in cell growth and the retinoic acid pathway. Wang ZG1, Delva L, Gaboli M, Rivi R, Giorgio M, Cordon-Cardo C, Grosveld F, Pandolfi PP. PMID 9488655 [Pubmed Abstract]

Sponsor: NCI Harvard SPORE in breast cancer 2P50 CA74031, NIH/NCI R01CA071692