Available Strain Details

Strain Information
Common Strain Name: Akt1, MPAKT Pricing and Ordering Information
Strain Nomenclature: FVB-Tg(Pbsn-AKT1)9Wrs/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: Akt Expression and Activation is sufficient for the induction of prostate intraepithelial neoplasia (PIN). Histologic study of the ventral prostate of the Murine Prostate restricted Akt Kinase Transgenic mice (MPAKT) revealed a striking phenotype characterized by a hyperplastic and dysplastic epithelium with disorganized multi-cell layers, intraepithelial lumen formation, loss of cell polarity, nuclear atypia and apoptotic bodies. These histopathologic features are consistent with PIN.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: Mouse thymoma viral proto-oncogene 1
Transgene Symbol: Akt1
Promotor Name: Probasin
Promotor Symbol: Pb
Current Genetic Background: FVB
Approx. Generation: 16
Organ Site: Prostate
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB/N females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype FVB/N (male or female).
Genotyping Information: Protocol 1: Allele: Tg(Pb-Akt1)Ak9Wrs
Donating Investigator: William R. Sellers

Key Reference: Majumder PK, Yeh JJ, George DJ, Febbo PG, Kum J, Xue Q, Bikoff R, Ma H, Kantoff PW, Golub TR, Loda M, Sellers WA. Prostate intraepithelial neoplasia induced by prostate restricted Akt activation: the MPAKT model. Proc Natl Acad Sci USA 100, 7841-7846, 2003. PMID: 12799464. [Pubmed Abstract]