Available Strain Details

Strain Information
Common Strain Name: Aprt Pricing and Ordering Information
Strain Nomenclature: 129S-Aprttm1Jat/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: Male and female Aprt(+/neo) or Aprtneo/neo mutant mice of 129/Sv background are crossed to wild-type mice of another strain to generate Aprt(+/neo) F1 hybrids. With time, LOH occurs at the Aprt locus in some cells. These cells can be isolated through selection for the loss of Aprt. By analyzing Aprt gene sequence, simple sequence repeat markers flanking the Aprt locus on chromosome 8, and cytological markers, it is possible to assign each somatic cell variant to a particular LOH pathway. The Aprt heterozygous model has been extremely useful in dissection of mutational pathways in vivo and in stem cells. Among the findings is that loss of heterozygosity (LOH) is the predominant pathway to mutation in vivo but is suppressed by about two orders of magnitude in stem cells.
Mutation Information
Mutation Type: Targeted
Gene Name: adenine phosphoribosyltransferase
Gene Symbol: Aprt
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: 129/Sv
Approx. Generation:
Organ Site: All
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is 129S1/SvlmJ/Cr females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype 129 (male or female).
Genotyping Information: Protocol 1: Allele: Aprt<tm1Jat>
Donating Investigator: Dr. Peter J. Stambrook

Key Reference: Shao, C., Deng, L., Henegariu,O., Liang, L., Raikwar, N., Sahota, A., Stambrook, P.J. & Tischfield, J. A. (1999) Mitotic recombination produces the majority of recessive fibroblast variants in heterozygous mice. Proc. Natl. Acad. Sci. U.S.A 96:9230-9235. PMID: 10430925 [Pubmed Abstract]

Related Reference: Liang L, Deng L, Shao C, Stambrook PJ, Tischfield JA.In vivo loss of heterozygosity in T-cells of B6C3F1 Aprt(+/-) mice. Environ Mol Mutagen. 2000;35(2):150-7. PMID: 10712749 [Pubmed Abstract]

Shao C, Deng L, Henegariu O, Liang L, Stambrook PJ, Tischfield JA. Chromosome instability contributes to loss of heterozygosity in mice lacking p53. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7405-10. PMID: 10861008 [Pubmed Abstract]

Shao C, Stambrook PJ, Tischfield JA. Mitotic recombination is suppressed by chromosomal divergence in hybrids of distantly related mouse strains. Nat Genet. 2001 Jun;28(2):169-72. PMID: 11381266 [Pubmed Abstract]