Available Strain Details

Strain Information
Common Strain Name: HB-PLAP(A to G) Pricing and Ordering Information
Strain Nomenclature: FVB-Tg(ACTB-ALPP)03Jrst/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): C1

Strain Description: Mutant plap gene with A to G transition mutation in a spice acceptor site resulting in a non-functional plap gene product. The transgenic mouse reports G to A transition mutations by reverting the plap transgene to wildtype. Histological assay of heat stable alkaline phosphatase activity detects G to A transition mutations in all tissues of the animal. Useful for detecting mutation distribution resulting from genetic defects and environmental exposure to mutagens.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: human placental alkaline phosphatase
Transgene Symbol: PLAP
Promotor Name: human beta actin
Promotor Symbol: ACTB
Current Genetic Background: FVB
Approx. Generation:
Organ Site:
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB/N Females x homozygous males. When maintained as a live colony, breeder pairs were distributed as homozygotes.
Genotyping Information: Protocol 1: Allele: Tg(ACTB-ALPP)03Jrst
Protocol 2: Allele: Tg(ACTB-ALPP)03Jrst
Donating Investigator: Dr. James Stringer

Key Reference: DePrimo SE, Cao J, Hersh MN, and Stringer JR. 1998 Use of human placental alkaline phosphatase transgenes to detect somatic mutation in mice in situ. Methods 16: 49-61. PMID: 9774516 [Pubmed Abstract]