Available Strain Details

Strain Information
Common Strain Name: p19(Arf) null (FVB) Pricing and Ordering Information
Strain Nomenclature: FVB.129-Cdkn2atm1Nesh/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): A

Strain Description: This is a KO of exon 1 beta of p19Arf (the p19Arf-specific KO), an important tumor suppressor gene. This allele is similar to a p19Arf KO made by the Sherr lab (Cdkn2atm1Cjs), except this KO deletes the selectable marker (Neo). These animals may be slightly less tumor prone than the Sherr KO, and do not overexpress p16Ink4a (the other product of the Ink4a/Arf locus). These animals were made in a manner identical to the p16Ink4a-specific KO available from the NCI Mouse Repository, and serve as useful comparison to that strain.
Mutation Information
Mutation Type: Targeted
Gene Name: Cyclin-dependent kinase inhibitor 2A
Gene Symbol: Cdkn2a
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: FVB.129
Approx. Generation: 7
Organ Site: All
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype FVB (male or female).
Genotyping Information: Protocol 1: Allele: Cdkn2a<tm1Nesh>
Donating Investigator: Dr. Norman Sharpless

Key Reference: Sharpless NE, Ramsey MR, Balasubramanian P, Castrillon DH, DePinho RA (2004). The differential impact of p16(INK4a) or p19(ARF) deficiency on cell growth and tumorigenesis. Oncogene 23, 379-385. PMID: 14724566 [Pubmed Abstract]

Related Reference: Kannan K, Sharpless NE, Xu J, O'Hagan RC, Bosenberg M, Chin L. Components of the Rb pathway are critical targets of UV mutagenesis in a murine melanoma model. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1221-5. Epub 2003 Jan 21. PMID: 12538879 [Pubmed Abstract]