Available Strain Details

Strain Information
Common Strain Name: EGFR-L858R Pricing and Ordering Information
Strain Nomenclature: B6;CBA-Tg(tetO-EGFR*L858R)56Hev/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B1

Strain Description: These mice need to be crossed to an appropriate rtTA strain for inducible expression of the EGFR L858R allele. EGFR L858R has been found in lung tumors from patients who respond to the tyrosine kinase inhibitors erlotinib and gefitinib. In double transgenic mice, expression of this mutant in the lung gave rise to lung tumors that required continued EGFR Del L747-S752 expression for survival and regressed when treated with erlotinib (Politi et al., 2006).
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: EGFR-L858R point mutation
Transgene Symbol: EGFR-L858R
Promotor Name: Tet-inducible promotor
Promotor Symbol: tetO
Current Genetic Background: B6;CBA
Approx. Generation: 21
Organ Site: Various, Lung
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x hemizygous males. SPERM: Sperm was obtained from hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype C57BL/6 (male or female). Average litter size is 10.
Genotyping Information: Protocol 1: Allele: Tg(tetO-EGFR*L858)56Hev
Donating Investigator: Dr. Katerina Politi

Key Reference: Politi K, Zakowski MF, Fan PD, Schonfeld EA, Pao W, Varmus HE. Lung adenocarcinomas induced in mice by mutant EGFR receptors found in human lung cancers respond to a tyrosine kinase inhibitor or to down-regulation of the receptors. Genes & Development 20, 1496-1510, 2006. PMID:16705038 [Pubmed Abstract]

Sponsor: Small Animal Imaging Resource Program [SAIRP] grant ]NIH R24CA83084] to Jason Koutcher, American Cancer Society- Post-doctoral Fellowship (PF-05-078-01-MGO) to Katerina Politi, Labrecque Foundation, NCI (K08-CA097980) to William Pao, Carmel Hill Fund, Joan's Legacy