Available Strain Details

Strain Information
Common Strain Name: RIP1-Tag5 Pricing and Ordering Information
Strain Nomenclature: C3H/He-Tg(RIP1-Tag5)5Dh/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): A

Strain Description: RIP1-TAg5 mice show a delayed onset of SV49 large T transgene expression, with sporadic activation of expression in a subset of the pancreatic beta cells beginning at 9-10 weeks of age. Tumors develop by 24 weeks with 100% penetrance. An autoimmune response develops against the oncogene-expressing beta cells, unlike in the RIP-TAg2 strain where mice are systemically tolerant to large T antigen. This strain presents a more typical tumor phenotype, in that not all beta cells express the transgene and the expression is activated in adulthood.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: SV40 Large T antigen
Transgene Symbol: TAg
Promotor Name: Rat Insulin Promoter - SV40 Large T antigen
Promotor Symbol: RIP1-Tag5
Current Genetic Background: C3HeB/Fe
Approx. Generation: 12
Organ Site: GI
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C3H/HeJCr females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x C3H/HeNCr (MTV -) (male or female). This strain was maintained as a continuous backcross to C3HeB/FeJ prior to arriving at the MMHCC Repository and is now maintained as a continuous backcross to C3H/HeN (MTV -). Neither C3H strains carry MTV (mouse mammary tumor virus). This strain originally arose from the microinjection of B6D2F2 embryos. Wild type littermates may be used in the breeding scheme.
Genotyping Information: Protocol 1: Allele: Tg(RIP1-Tag5)5Dh
Donating Investigator: Dr. Doug Hanahan

Key Reference: Hanahan D. 1985. Heritable formation of pancreatic beta-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes. Nature 315:115-22 PMID: 2986015 [Pubmed Abstract]