Available Strain Details

Strain Information
Common Strain Name: RosaSB Pricing and Ordering Information
Strain Nomenclature: B6;129-Gt(ROSA)26Sortm1(sb11)Njen/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): C1

Strain Description: To avoid epigenetic silencing often seen with transgenic insertions, the SB11 transposase was knocked into the ROSA26 locus (RosaSB). Relative to SB10, SB11 contains four amino acid substitutions that increase enzymatic activity. Expression of SB11 in RosaSB mice was equal in all tissues tested (brain, spleen, skin, lung). This strain is part of the improved Sleeping Beauty mutagenesis system developed by Nancy Jenkins' laboratory.
Mutation Information
Mutation Type: Targeted
Gene Name: ROSA26
Gene Symbol: ROSA26
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6;129
Approx. Generation: 10
Organ Site: Various
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. SPERM: Sperm was obtained from heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female). Average litter size is 6-7.
Genotyping Information: Protocol 1: Allele: Gt(ROSA)26Sor<tm1(sb11)Njen
Donating Investigator: Dr. Adam Dupuy

Key Reference: Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature 436, 221-226, 2005. PMID:16015321 [Pubmed Abstract]

Related Reference: Geurts AM; Collier LS; Geurts JL; Oseth LL; Bell ML; Mu D; Lucito R; Godbout SA; Green LE; Lowe SW; Hirsch BA; Leinwand LA; Largaespada DA. Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers. PLoS Genet 2, e156, 2006. PMID:17009875 [Pubmed Abstract]