Available Strain Details

Strain Information
Common Strain Name: TG6070 Pricing and Ordering Information
Strain Nomenclature: B6;C3-TgTn(sb-T2/Onc2)6070Njen/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): C1

Strain Description: The Sleeping Beauty mutagenic transposon [Tn(sb-T2/Onc2); T2/Onc2] was engineered to contain an engrailed-2 splice acceptor flanked by SB transposase binding sites that were optimized to increase transposition frequency. The transposon also contained splice acceptors and a bi-directional polyadenylation signal that can terminate transcription when integrated in either orientation into a gene. In addition, a murine stem cell virus (MSCV) long terminal repeat (LTR) and a splice donor were included to activate gene expression when integrated upstream of a gene. Two strains of transgenic T2/Onc2 mice were generated and screened for high transgene copy number and lack of transgene methylation. Strain 6070 [B6;C3- TgTn(sb-T2/Onc2)6070Njen] contained a transposon concatemer on chromosome 4.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: T2/Onc2 transposon (Sleeping Beauty)
Transgene Symbol: T2/Onc2
Promotor Name: MSCV LTR (viral)
Promotor Symbol: MSCV LTR
Current Genetic Background: B6;C3
Approx. Generation: 10
Organ Site: Various
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x hemizygous males. SPERM: Sperm was obtained from hemizgyous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype C57BL/6 (male or female). Average litter size is 8.
Genotyping Information: Protocol 1: Allele: TgTn(sb-T2/Onc2)6070Njen
Donating Investigator: Dr. Adam Dupuy

Key Reference: Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature 436, 221-226, 2005. PMID:16015321 [Pubmed Abstract]

Related Reference: Geurts AM; Collier LS; Geurts JL; Oseth LL; Bell ML; Mu D; Lucito R; Godbout SA; Green LE; Lowe SW; Hirsch BA; Leinwand LA; Largaespada DA. Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers. PLoS Genet 2, e156, 2006. PMID:17009875. [Pubmed Abstract]

Sponsor: National Cancer Institute