Available Strain Details

Strain Information
Common Strain Name: C57BL/6 DNTGFRII Pricing and Ordering Information
Strain Nomenclature: C57BL/6-Tg(CD2-Tgfbr2)1Grs/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: These transgenic mice express a dominant-negative form of the TGF beta type II receptor (DNRII) under control of the human CD2 promoter/enhancer. Both CD4+ and CD8+ T-cells expressed comparable amounts of the transgene, whereas no transgene mRNA was detected in T cell-depleted lymphocyte populations (Lucas et al., 2000). DNRII expression resulted in clonal expansion and progressive hyperproliferation of CD8+ memory T-cells, resulting in dramatically increased leukocyte counts by 30 weeks of age. Homozygous mice exhibited symptoms of decreased activity, loss of weight, and difficulty breathing 4 months before hemizygous animals became symptomatic (Lucas et al., 2004). The disease progressed to leukemia/lymphoma as determined by histology, T-cell receptor monoclonality, and host transferability.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: transforming growth factor, beta receptor II
Transgene Symbol: Tgfbr2
Promotor Name:
Promotor Symbol:
Current Genetic Background: C57BL/6
Approx. Generation: 5
Organ Site: Hematopoietic
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype C57BL/6 (male or female). Average litter size is 6. Breeders need to be kept young (< 5 mo).
Genotyping Information: Protocol 1: Allele: Tg(CD2-Tgfbr2)1Grs
Donating Investigator: Philip Lucas

Key Reference: Lucas PJ, Kim S-J, Melby SJ, Gress RE. Disruption of T-cell homeostasis in mice expressing a T cell-specific dominant negative transforming growth factor beta II receptor. J Exp Med 191, 1187-1196, 2000. PMID: 10748236. [Pubmed Abstract]

Related Reference: Lucas PJ, McNeil N, Hilgenfeld E, Choudhury B, Kim S-J, Eckhaus MA, Ried T, Gress RE. Transforming growth factor beta pathway serves as a primary tumor suppressor in CD8+ T-cell tumorigenesis. Cancer Res 64, 6524-6529, 2004. PMID: 15374963. [Pubmed Abstract]

Sponsor: The National Institutes of Health, National Cancer Institute, ACS