Available Strain Details

Strain Information
Common Strain Name: Tyr-HRAS Pricing and Ordering Information
Strain Nomenclature: FVB/N-Tg(Tyr-HRAS)60Lc/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: This strain carries an activated human HRAS gene (G12V) that is expressed in melanocytes. The transgene integrated on the mouse Y chromosome and therefore, only males develop tumors. On an Ink4a/Arf null background, spontaneous cutaneous tumors develop most commonly in the pinna of the ear, torso, tail, and perineum area. Ocular tumors are seen occasionally. Tumors are locally invasive with no evidence of metastasis. On a wild-type Ink4a/Arf background, there is a very low tumor frequency after a long latency.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: RAS oncogene
Transgene Symbol: HRAS
Promotor Name: Tyrosinase enhancer/promoter element
Promotor Symbol: Tyr
Current Genetic Background: FVB/N
Approx. Generation: 18
Organ Site: Skin
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB/N females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote male x wildtype FVB/N female.
Genotyping Information: Protocol 1: Allele: Tg(Tyr-HRAS)60Lc
Donating Investigator: Dr. Lynda Chin

Key Reference: Lynda Chin, Jason Pomerantz, David Polsky, Mark Jacobson, Carlos Cohen, Carlos Cordon-Cardo, James W. Horner, II, and Ronald A. DePinho. 1997. Cooperative effects of INK4a and ras in melanoma susceptibility in vivo. Genes Dev. 11: 2822-2834. PMID: 9353252 [Pubmed Abstract]