Available Strain Details

Strain Information
Common Strain Name: TetO-HRAS Pricing and Ordering Information
Strain Nomenclature: FVB-Tg(tetO-HRAS)65Lc/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: This strain carries an activated HRAS human gene (G12V) under the control of a minimal promoter containing multimerized tet-operons (TetO). TetO will drive expression of the HRAS transgene in the presence of a tetracycline transcriptional activator(tTA), or a tetracycline reverse transcriptional activator (rtTA), in the absence or presence of doxycycline (Dox), respectively. In normal use this strain would be mated with a transgenic strain carrying tTA or rtTA under the control of a tissue specific promoter/enhancer.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: RAS oncogene
Transgene Symbol: HRAS
Promotor Name: Tet Operon
Promotor Symbol: TetO
Current Genetic Background: FVB
Approx. Generation: 16
Organ Site: All
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB/N females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype FVB/N (male or female).
Genotyping Information: Protocol 1: Allele: Tg(TetO-HRAS)65Lc
Donating Investigator: Dr. Lynda Chin

Key Reference: Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, Shen Q et.al. 1999. Essential role for oncogenic Ras in Tumour maintenance. Nature 400:468-72. PMID: 10440378 [Pubmed Abstract]