Available Strain Details

Strain Information
Common Strain Name: MMTV-Cre, Line F Pricing and Ordering Information
Strain Nomenclature: B6.Cg-Tg(MMTV-Cre)FMam/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): C1

Strain Description: Widespread Cre expression including the female germline. High levels of recombination in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin and cells of the immune system. Little expression in the lung, kidney, liver and brain. Females from this line display impaired mammary development and are unable to nurse successfully, so if used in the colony pups must be fostered. Refer to PMID 21255551 for details.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: Cre recombinase
Transgene Symbol: Cre
Promotor Name: Mouse mammary tumor virus
Promotor Symbol: MMTV
Current Genetic Background: B6.Cg
Approx. Generation: 28
Organ Site: Breast
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype C57BL/6 (male or female). Females from this line display impaired mammary development and are unable to nurse successfully. Refer to PMID: 21255551 for details.
Genotyping Information: Protocol 2: Allele: Tg(MMTV-Cre)FMam
Donating Investigator: Dr. Lothar Hennighausen

Key Reference: Wagner KU, Wall RJ, St-Onge L, Gruss P, Wynshaw-Boris A, Garrett L, Li M, Furth PA, Hennighausen L 1997. Cre-mediated gene deletion in the mammary gland. Nucleic Acids Res 25 :4323-4330. PMID: 9336464 [Pubmed Abstract]

Related Reference: Robinson GW, Hennighausen L. MMTV-Cre transgenes can adversely affect lactation: Considerations for conditional gene deletion in mammary tissue. Anal Biochem. 2011 Jan 19. PMID: 21255551 [Pubmed Abstract]