Available Strain Details

Strain Information
Common Strain Name: Tgfb1 Rag2 null Pricing and Ordering Information
Strain Nomenclature: STOCK Tgfb1tm1Doe Rag2tm1Fwa/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: Tgfb1-/- Rag2-/- mice rapidly develop mucosal hyperplasia of the cecum and colon which progresses to adenoma and carcinoma. All mice are affected with multiple tumors by 5 months of age. Ceca and colon from Tgfb1+/+ Rag2-/- and Tgfb1+/- Rag2-/- exhibit hyperplasia, which in most cases does not develop further. There are no differences in cell proliferation, inflammation, or apoptosis in hyperplasic tissues from the three genotypes, indicating that the conversion to adenoma is a result of the inability to maintain epithelial tissue organization as opposed to loss of growth control, increased inflammatory activity or increased genetic instability.
Mutation Information
Mutation Type: Targeted
Gene Name: Transforming growth factor, beta 1
Gene Symbol: Tgfb1
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: STOCK
Approx. Generation: 52
Organ Site: GI
Additional Information
Cryopreservation Mating Scheme: When maintained as a live colony, breeder pairs were distributed as non-sibling breeders - Tgfb1(+/-) Rag2(-/-) x Tgfb1(+/+) Rag2(-/-). Non-sibling matings are recommended to avoid inbreeding.
Genotyping Information: Protocol 1: Allele: Rag2<tm1Fwa>
Protocol 1: Allele: Tgfb1<tm1Doe>
Donating Investigator: Dr. Tom Doetschman

Key Reference: Engle SJ, Hoying JB, Boivin GP, Ormsby I, Gartside PS, Doetschman T. 1999. Transforming growth factor beta1 suppresses nonmetastatic colon cancer at an early stage of tumorigenesis. Cancer Res. 59:3379-86. PMID: 10416598 [Pubmed Abstract]

Related Reference: Engle SJ1, Ormsby I, Pawlowski S, Boivin GP, Croft J, Balish E, Doetschman T. Elimination of colon cancer in germ-free transforming growth factor beta 1-deficient mice. Cancer Res. 2002 Nov 15;62(22):6362-6. PMID: 12438215 [Pubmed Abstract]

Makwana M1, Jones LL, Cuthill D, Heuer H, Bohatschek M, Hristova M, Friedrichsen S, Ormsby I, Bueringer D, Koppius A, Bauer K, Doetschman T, Raivich G Endogenous transforming growth factor beta 1 suppresses inflammation and promotes survival in adult CNS. J Neurosci. 2007 Oct 17;27(42):11201-13. PMID: 17942715 PMCID: PMC6673043 [Pubmed Abstract]