Available Strain Details

Strain Information
Common Strain Name: Mlh1 null Pricing and Ordering Information
Strain Nomenclature: B6.129-Mlh1tm1Rak/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B

Strain Description: This strain carries a null mutation in the DNA mismatch repair gene Mlh1. Cells from mice homozygous for this mutation are incapable of repairing single-nucleotide and insertion/deletion mismatches. Homozygous males have no detectable mature sperm and are sterile. Homozygous female mice have normal estrous cycles but are also sterile. Both males and females exhibit normal mating behavior. Heterozygous and homozygous mice show cancer susceptibility.
Mutation Information
Mutation Type: Targeted
Gene Name: MutL homolog 1
Gene Symbol: Mlh1
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129
Approx. Generation: 15
Organ Site: GI
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. SPERM: Sperm was obtained from heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female). Mutants are generated by mating heterozygous pairs.
Genotyping Information: Protocol 1: Allele: Mlh1<tm1Rak>
Donating Investigator: Dr. Raju Kucherlapati

Key Reference: Edelmann W, Cohen PE, Kane M, Lau K, Morrow B, Bennett S, Umar A, Kunkel T, Cattoretti G, Chaganti R, Pollard JW, Kolodner RD, Kucherlapati R. Meiotic pachytene arrest in MLH1-deficient mice. 1996. Cell 85:1125-34. PMID: 8674118 [Pubmed Abstract]