Available Strain Details

Strain Information
Common Strain Name: CAG-LSL-EGFR-WT Pricing and Ordering Information
Strain Nomenclature: STOCK Col1a1tm1(CAG-EGFR)Char/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B3

Strain Description: None until presented to tissue specific Cre recombinase.
Mutation Information
Mutation Type: Targeted
Gene Name: Wild type epidermal growth factor receptor
Gene Symbol: EGFR
Transgene Name:
Transgene Symbol:
Promotor Name: CAGGS (chicken and viral)
Promotor Symbol: CAGGS
Current Genetic Background: STOCK
Approx. Generation: 7
Organ Site: Any
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype FVB (male or female). Average litter size is 7.
Genotyping Information: Protocol 1: Allele: Col1a1<tm1(CAG-EGFR)Char>
Donating Investigator: Dr. Alain Charest

Key Reference: Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2712-6. doi: 10.1073/pnas.0813314106. Epub 2009 Feb 5., Oncogenic EGFR signaling cooperates with loss of tumor suppressor gene functions in gliomagenesis., Zhu H1, Acquaviva J, Ramachandran P, Boskovitz A, Woolfenden S, Pfannl R, Bronson RT, Chen JW, Weissleder R, Housman DE, Charest A. PMID: 19196966 [PubMed - indexed for MEDLINE]PMCID: PMC2650331 [Pubmed Abstract]

Related Reference: Stem Cells. 2014 Feb;32(2):338-48. doi: 10.1002/stem.1554. Inhibition of EGFR induces a c-MET-driven stem cell population in glioblastoma. Jun HJ1, Bronson RT, Charest A, PMID: 24115218 PMCID: PMC4442493 DOI: 10.1002/stem.1554 [Pubmed Abstract]

Oncogene. 2012 Jun 21;31(25):3039-50. doi: 10.1038/onc.2011.474. Epub 2011 Oct 24. Acquired MET expression confers resistance to EGFR inhibition in a mouse model of glioblastoma multiforme. Jun HJ1, Acquaviva J, Chi D, Lessard J, Zhu H, Woolfenden S, Bronson RT, Pfannl R, White F, Housman DE, Iyer L, Whittaker CA, Boskovitz A, Raval A, Charest A., PMID: 22020333 PMCID: PMC3774279 DOI: 10.1038/onc.2011.474 [Pubmed Abstract]

Cancer Res. 2011 Dec 1;71(23):7198-206. doi: 10.1158/0008-5472.CAN-11-1514. Epub 2011 Oct 10. Chronic activation of wild-type epidermal growth factor receptor and loss of Cdkn2a cause mouse glioblastoma formation. Acquaviva J1, Jun HJ, Lessard J, Ruiz R, Zhu H, Donovan M, Woolfenden S, Boskovitz A, Raval A, Bronson RT, Pfannl R, Whittaker CA, Housman DE, Charest A., PMID: 21987724 PMCID: PMC3228869 DOI: 10.1158/0008-5472.CAN-11-1514 [Indexed for MEDLINE] Free PMC Article [Pubmed Abstract]

Sponsor: National Institutes of Health U54 CA119349, U01 CA141556