Available Strain Details

Strain Information
Common Strain Name: Arf floxed Pricing and Ordering Information
Strain Nomenclature: B6.129-Cdkn2atm4Cjs/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B1

Strain Description: Arf-null mice are highly tumor prone. 80% succumb of cancer (43 % of sarcoma, 29% of lymphoma, 17% of carcinoma and 11% of tumors of the nervous system) by 12 months of age (mean latency for survival of 38 weeks) (Kamijo et al, Cancer Res 1999, 59: 2217-2222). The latency and the penetrance are shortened when the mice are irradiated, or treated with DMBA (Kamijo et al, Cancer Res 1999, 59: 2217-2222). To prevent unwanted tumorigenesis outside of the tissue of interest, we generated an Arf floxed line. By crossing this line with a transgenic line expressing Cre recombinase in specific tissues and/ or in an inducible manner, Arf could be deleted in specific tissues and/ or spatially and temporally
Mutation Information
Mutation Type: Targeted
Gene Name: Cyclin-dependent kinase inhibitor 2A
Gene Symbol: Cdkn2a
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129
Approx. Generation: HOMO
Organ Site:
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is homozygous females x homozygous males. SPERM: Sperm was obtained from homozgyous males. When maintained as a live colony, breeder pairs were distributed as homozygotes. Average litter size is 8-10.
Genotyping Information: Protocol 1: Allele: Cdkn2a<tm4Cjs>
Donating Investigator: Dr. Charles Sherr

Key Reference: Gromley A, Churchman ML, Zindy F, Sherr CJ. Howard Hughes Medical Institute and Department of Genetics & Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. PMID: 19339492 [Pubmed Abstract]

Sponsor: HHMI, St. Jude Children's Research Hospital