Available Strain Details

Strain Information
Common Strain Name: WAPT121 Pricing and Ordering Information
Strain Nomenclature: B6D2F1-Tg(WAPT121)66Tvd/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): C1

Strain Description: WAPT121 transgenic mice express T121, a trucation mutation of SV40 antigen that binds and inactivates all pRB-related proteins, under control of whey acidic protein (WAP) promoter. T121 expression was detected in the mammary gland (Simin et al., 2004). Transgenic females failed to nurse pups because of lactation defects. Multi-pregnant females developed mammary tumors at 11-15 months after the first pregnancy. Tumorigenesis is accelerated 2-fold on a Trp53 heterozygous background.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: T121
Transgene Symbol: T121
Promotor Name: WAP
Promotor Symbol: WAP
Current Genetic Background: B6D2
Approx. Generation:
Organ Site:
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is B6D2F1 females x heterozygous males. Females do not nurse young so can't be used for breeding. When maintained as a live colony, breeder pairs were distributed as a hemizygote male x wildtype B6D2F1 female. Average litter size is 9.
Genotyping Information: Protocol 1: Allele: Tg(WAPT121)66Tvd
Donating Investigator: Dr. Terry Van Dyke

Key Reference: Simin K, Wu H, Lu L, Pinkel D, Albertson D, Cardiff RD, Van Dyke T. pRb inactivation in mammary cells reveals common mechanisms for tumor initiation and progression in divergent epithelia. PLoS Biol. 2004Feb; 2(2). PMID: 14966529 [Pubmed Abstract]

Related Reference: Herschkowitz JI, Simin K, Weigman VJ, Mikaelian I, Usary J, Hu Z, Rasmussen KE, Jones LP, Assefnia S, Chandrasekharan S, Backlund MG, Yin Y, Khramtsov AI, Bastein R, Quackenbush J, Glazer RI, Brown PH, Green JE, Kopelovich L, Furth PA, Palazzo JP, Olopade OI, Bernard PS, Churchill GA, Van Dyke T, Perou CM. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 2007 May 10;8(5):R76. PMID: 17493263 [Pubmed Abstract]

Sponsor: NIH 5-RO1-CA046283-16, Susan G. Komen 9864, USAMR DAMD17-99-1-9332