Available Strain Details

Strain Information
Common Strain Name: Gli-Luc/FVB Pricing and Ordering Information
Strain Nomenclature: STOCK Tg(CRYD/Gli-luc)#Ech/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): C1

Strain Description: The Gli-luc reporter mouse was generated using an eight GLI consensus sequence; it is an invivo tumor model to noninvasively image Gli activity. We demonstrated that Gli activity, measured by luciferase bioluminescence (BLI), correlates with tumor grade. The regions of Gli activity can be visualized after injection of luciferase substrate to give an accurate readout of Gli activation, providing visualization of tumor size and location. This mouse line, termed Gli-luc, expresses luciferase from a Gli responsive promoter activated by Gli, a primary target of SHH signaling. See references
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: luciferase
Transgene Symbol: luciferase
Promotor Name: synthetic eight Gli DNA upstream of chicken lens delta-crystallin basal
Promotor Symbol:
Current Genetic Background: STOCK
Approx. Generation:
Organ Site:
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: Cryopreservation mating scheme is (Tg/Tg) male x (Tg/Tg) female OR (Tg/+) male x (Tg/+) female; therefore, progeny is expected to be transgenic OR wildtype, so must be genotyped. SPERM: Sperm was obtained from (Tg/+) males.
Genotyping Information: Protocol 1: Allele: Tg(CRYD/Gli-luc)#Ech/Nci
Donating Investigator: Dr. Eric Holland

Key Reference: Becher OJ, et al., Gli activity correlates with tumor grade in platelet-derived growth factor-induced gliomas. Cancer Res. 2008 Apr 1;68(7):2241-9; PMID: 18381430 MGI:4820828 J:133329 [Pubmed Abstract]

Sponsor: NIH ROI CA 5R01CA099489-1, ROI 5RO1CA100688-2, ROI CA?NS96582-01 and Kirby Foundation