Available Strain Details

Strain Information
Common Strain Name: RARa-PML Pricing and Ordering Information
Strain Nomenclature: C3;B6-Tg(CTSG-RARa/PML)2683Ley/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: This strain carries an alternatively spliced bcr-3 isoform of RARa-PML under the control of sequences from the human cathepsin G gene (hCG), which directs expression of the transgene to promyelocytes. The source of the transgene is a human cDNA found in patients with acute promyelocytic leukemia (APML). Expression of the transgene does not alter the development of myeloid cells nor does it cause APML. However, in combination with the bcr-1 transgene (PML-RARa, strain hCG-PML-RARa line 135) the penetrance of APML is increased from 15% in the PML-RARa strain to 57% for the double transgenic. The 6-18 month latency in the development of APML is not altered. The leukemias that develop in mice carrying PML-RARa alone, or in combination with RARa-PML, respond to treatment with all-trans retinoic acid (ATRA).
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: Retinoic acid receptor alpha, Promyelocytic leukemia (human)
Transgene Symbol: RARa-PML
Promotor Name: Cathespin G (human)
Promotor Symbol: hCG
Current Genetic Background: C3;B6
Approx. Generation:
Organ Site: Hematopoietic
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is B6C3F1/Cr females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C3B6F1 (male or female).
Genotyping Information: Protocol 1: Allele: Tg(CTSG-RARA/PML)2683Ley
Donating Investigator: Dr. Timothy Ley

Key Reference: Pollock JL, Westervelt P, Kurichety AK, Pelicci PG, Grisolano JL, Ley TJ. 1999 A bcr-3 isoform of RARa-PML potentiates the development of PML-RARa-driven acute promyelocytic leukemia. PNAS 96:15103-108. PMID: 10611345 [Pubmed Abstract]