Available Strain Details

Strain Information
Common Strain Name: PML-RARa Pricing and Ordering Information
Strain Nomenclature: C3;B6-Tg(CTSG-PML/RARa)135Ley/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: This strain carries a chimeric promyelocytic leukemia-retinoic acid receptor alpha transgene (PML-RARa) under the control of sequences from the human cathepsin G gene, which directs expression of the transgene to promyelocytes. The source of the transgene is a human cDNA generated as a result of a specific t(15;17) chromosomal translocation found in most humans cases of acute promyelocytic leukemia (APML). Increased percentages of immature and mature myeloid cells are found in the peripheral blood, bone marrow and spleen. Approximately 15% of the transgenic mice develop acute myeloid leukemia after a long latency (starting around 6 months of age). Splenic promyelocytes and intact mice respond to all-trans retinoic acid (ATRA). The penetrance of APML increases to about 60% when this strain is mated to the strain carrying a bcr-3 isoform of RARa-PML (hCG-RARa-PML).
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: Promyelocytic leukemia, Retinoic acid receptor alpha (human)
Transgene Symbol: PML/RARA
Promotor Name: Cathepsin G (human)
Promotor Symbol: hCG
Current Genetic Background: C3;B6
Approx. Generation:
Organ Site: Hematopoietic
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is B6C3F1/Cr females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C3B6F1 (male or female).
Genotyping Information: Protocol 1: Allele: Tg(CTSG-PML/RARA)135Ley
Donating Investigator: Dr. Timothy Ley

Key Reference: Grisolano JL, Wesselschmidt RL, Pelicci PG, Ley TJ. 1997. Altered myeloid development and acute leukemia in transgenic mice expressing PML-RARa under control of cathepsin G regulatory sequences. Blood 89:376-387. PMID: 9002938 [Pubmed Abstract]