Available Strain Details

Strain Information
Common Strain Name: K-rasLA2 Pricing and Ordering Information
Strain Nomenclature: B6.129S-Krastm3Tyj/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B3

Strain Description: This strain carries a targeted latent 'hit-and-run' K-ras allele that can be activated by an in vivo spontaneous recombination event ('run'). The in vivo recombination event results in an activated allele (K-rasG12D). Mice carrying this mutation have a mean age of death/sacrifice of 200 days as a result of extensive tumor burden. The most frequent organ site is the lung. Varying grades of tumors are present from hyperplasia/dysplasia to carcinomas similar to human non-small cell lung cancer. Immunohistochemistry indicates tumors develop in alveolar type II cell lineage. Metastasis to thorasic lymph nodes, kidney and other visceral organs occurs with low frequency. Other organ sites include the thymus (thymic lymphoma) and skin (papillomas). A companion strain (K-rasLA1) carries an allele where one half of the in vivo recombination events result in a normal K-ras allele and one half in the K-rasG12D activated allele.
Mutation Information
Mutation Type: Targeted
Gene Name: Kirsten rat sarcoma oncogene, 2 expressed
Gene Symbol: Kras
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129S2
Approx. Generation: 42
Organ Site: Lung
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. SPERM: Sperm was obtained from heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female).
Genotyping Information: Protocol 1: Allele: Kras<tm3Tyj>
Donating Investigator: Dr. Tyler Jacks

Key Reference: Johnson L, Mercer K, Greenbaum D, Bronson RT, Crowley D, Tuveson DA, Jacks T. 2001. Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature.410:1111-6., PMID: 11323676 [Pubmed Abstract]

Related Reference: To MD1, Quigley DA, Mao JH, Del Rosario R, Hsu J, Hodgson G, Jacks T, Balmain A. Progressive genomic instability in the FVB/Kras(LA2) mouse model of lung cancer. Mol Cancer Res. 2011 Oct;9(10):1339-45. Epub 2011 Aug 1., PMID: 21807965 PMCID: PMC3196775 [Pubmed Abstract]

Young NP1, Jacks T. Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras., Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10184-9. Epub 2010 May 17. PMID: 20479239 PMCID: PMC2890471 [Pubmed Abstract]