Available Strain Details

Strain Information
Common Strain Name: K-rasLA1 Pricing and Ordering Information
Strain Nomenclature: B6.129S2-Krastm2Tyj/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B3

Strain Description: This strain carries a targeted latent 'hit-and-run' K-ras allele that can be activated by an in vivo spontaneous recombination event ('run'). One half of the in vivo recombination events result in a normal K-ras allele and one half in an activated allele (K-rasG12D). Mice carrying this mutation have a mean age of death/sacrifice of 300 days as a result of extensive tumor burden. The most frequent organ site is the lung. Varying grades of tumors are present from hyperplasia/dysplasia to carcinomas similar to human non-small cell lung cancer. Immunohistochemistry indicates tumors develop in alveolar type II cell lineage. Metastasis to thorasic lymph nodes, kidney and other visceral organs occurs with low frequency. Other organ sites include the thymus (thymic lymphoma) and skin (papillomas). A companion strain (K-rasLA2) carries an allele that recombines to the activated allele (K-rasG12D) 100% of the time.
Mutation Information
Mutation Type: Targeted
Gene Name: Kirsten rat sarcoma oncogene, 2 expressed
Gene Symbol: Kras
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129S2
Approx. Generation: 21
Organ Site: Lung
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. SPERM: Sperm was obtained from heterozygous males. When maintained as a live colony, breeder pairs were supplied as a heterozygote (male or female) x wildtype C57BL/6 (male or female).
Genotyping Information: Protocol 1: Allele: Kras<tm2Tyj>
Donating Investigator: Dr. Tyler Jacks

Key Reference: Johnson L, Mercer K, Greenbaum D, Bronson RT, Crowley D, Tuveson DA, Jacks. 2001. Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature 410:1111-6. PMID: 11323676 [Pubmed Abstract]

Related Reference: Huarte M1, Guttman M, Feldser D, Garber M, Koziol MJ, Kenzelmann-Broz D, Khalil AM, Zuk O, Amit I, Rabani M, Attardi LD, Regev A, Lander ES, Jacks T, Rinn JL. A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response. Cell. 2010 Aug 6;142(3):409-19. PMID: 20673990 PMCID: PMC2956184 [Pubmed Abstract]

DuPage M1, Cheung AF, Mazumdar C, Winslow MM, Bronson R, Schmidt LM, Crowley D, Chen J, Jacks T. Endogenous T cell responses to antigens expressed in lung adenocarcinomas delay malignant tumor progression. Cancer Cell. 2011 Jan 18;19(1):72-85. PMID: 21251614 PMCID: PMC3069809 [Pubmed Abstract]

Romero R1,2, Sayin VI3, Davidson SM1,2, Bauer MR1, Singh SX3, LeBoeuf SE3, Karakousi TR3, Ellis DC1,2, Bhutkar A1, Sánchez-Rivera FJ1,2, Subbaraj L1,2, Martinez B3, Bronson RT4,5, Prigge JR6, Schmidt EE6, Thomas CJ7, Goparaju C8, Davies A9, Dolgalev I10, Heguy A10, Allaj V11,12, Poirier JT11,12, Moreira AL3, Rudin CM11,12, Pass HI8, Vander Heiden MG1,2, Jacks T1,2,13, Papagiannakopoulos T3,14. Keap1 loss promotes Kras-driven lung cancer and results in dependence on glutaminolysis. Nat Med. 2017 Nov;23(11):1362-1368. Epub 2017 Oct 2., PMID: 28967920 PMCID: PMC5677540 [Pubmed Abstract]