Strain Protocols

Strain Information
Strain Code: 01XA2
Strain Name: B6.129-Mlh1tm1Rak/Nci
Gene Symbol: Mlh1
Protocol Information
Allele: Mlh1<tm1Rak>
Protocol Number: 1
Introductory Comment:


M001 : 5'-TGT cAA TAG GcT Gcc cTA GG-3'
M002 : 5'-TGG AAG GAT TGG AGc TAc GG-3'
M003 : 5'-TTT TcA GTG cAG ccT ATG cTc-3'

Product Sizes

Primer Combination Product Size
M001 / M002 500 bp    Knockout
M001 / M003 350 bp    Wildtype

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.5 mM
2.5mM dNTP Mix 0.2 mM
100 µMM001 0.33 µM
100 µMM002 0.33 µM
100 µMM003 0.3 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 1:00
65ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.