Strain Protocols

Strain Information
Strain Code: 01X68
Strain Name: STOCK Col1a1tm2(CAG-EGFR*)Char/Nci
Gene Symbol: EGFRvIII
Protocol Information
Allele: Col1a1<tm2(CAG-EGRF*)Char>
Protocol Number: 1
Introductory Comment:

Primers

67E1 : 5'-CCC CCT GAA CCT GAA ACA TAA-3'
68E3 : 5'-TAA ATG CCA CCG GCA GGA TG-3'

Product Sizes

Primer Combination Product Size
67E1 / 68E3 670 bp    MT

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2 mM
2.5mM dNTP Mix 0.2mM
100 µM67E1 0.5 µM
100 µM68E3 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.