Program Overview

The Antibody Characterization Laboratory is a reference program that develops antibodies to be made available to the scientific community, including extensive antibody-characterization data and protocols.

The laboratory uses an array of techniques to determine the properties of antibodies and other renewable reagents that can be used in cancer-related research. All of the reagents, associated data, and standard operating procedures are freely available as part of the National Cancer Institute’s Antibody Portal

Our scientists subject each antibody to a variety of assays and techniques that collectively reveal a full picture of its characteristics. We provide our approaches, standardized methods, and the complete characterization data (both positive and negative results). Our ready access to the NCI-60 human cancer cell lines makes our program a unique resource. 

The data we generate is designed to help researchers select the best antibodies for their experiments. They have access to our methods to perform quality control studies or biological assays. 

Program Innovations

To date, 783 antibodies have been developed to 470 antigen targets (mostly proteins and peptides). 

Program Focus

Antibody Portal

The Antibody Characterization Laboratory performs antibody validation as part of a community resource overseen by the National Cancer Institute Office of Cancer Clinical Proteomics Research.  

You may access our data, standards, and reference materials online and filter by your criteria: 

Characterization process and pipeline 

Our scientists produce up to three affinity reagents for each expressed and purified antigen, and one affinity reagent per peptide. We characterize selected reagents using specialized processes, including:   

  • ELISA 

  • Western blot 

  • Surface plasmon resonance 

  • Bio-Layer Interferometry 

  • Mass spectrometry immunoassay 

  • Immunohistochemistry 

  • NCI60 Protein Array