TypeSeqer HPV Genotyping

  • TypeSeqer assay workflow capable of individual detection of 52 HPV types, including all carcinogenic types.
  • Includes sufficient basic consultation with a subject matter expert.

Process

  • Perform a series of three polymerase chain reactions, with a dilution or enzyme treatment between the steps.
    • There are no individual sample purification or normalization steps required, allowing for a streamlined, high-throughput workflow with minimal hands-on time.
  • Run submitted samples on the Ion next-generation sequencing platforms.
    • Previously extracted DNA is acceptable for submission.
    • Submitted extracted DNA will be quantitatively assessed before final acceptance.
  • Provides automated reports containing assay performance and detected HPV types per sample.

Acceptance criteria

  • For DNA extraction service associated with this assay, the Cancer Genomics Research Laboratory requires 500 ul per sample in one of the following three sample media:
    • STM
    • Surepath
    •  PreservCyt

The assay has been designed to operate on Thermo Fisher Scientific Ion next-generation sequencing platforms as well as Illumina next-generation sequencing instruments.

Let's Talk

Amy Hutchinson Manager