TypeSeqer HPV Genotyping

• TypeSeqer assay workflow capable of individual detection of 52 HPV types, including all carcinogenic types.
• Includes sufficient basic consultation with a subject matter expert.

Process

• Perform a series of three polymerase chain reactions, with a dilution or enzyme treatment between the steps.
  • There are no individual sample purification or normalization steps required, allowing for a streamlined, high-throughput workflow with minimal hands-on time.
• Run submitted samples on the Ion next-generation sequencing platforms.
  • Previously extracted DNA is acceptable for submission.
  • Submitted extracted DNA will be quantitatively assessed before final acceptance.
• Provides automated reports containing assay performance and detected HPV types per sample.

Acceptance criteria

• For DNA extraction service associated with this assay, the Cancer Genomics Research Laboratory requires 500 ul per sample in one of the following three sample media:
  • STM
  • Surepath
  •  PreservCyt

The assay has been designed to operate on Thermo Fisher Scientific Ion next-generation sequencing platforms as well as Illumina next-generation sequencing instruments.