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Laboratory Animal Services

Pharmacokinetic Evaluation in Kras/p53 PDAC Mice

  • Detailed pathology assessment of H&E-stained tissues at endpoint.
  • Takes about five months to complete.
  •  The Laboratory Animal Sciences Program will provide a full toxicity report and a collection of H&E stained tissue sections (one slide per tissue block).
  • Includes sufficient basic consultation with a subject matter expert is included with the service.

Process

  • Breed 24 KPC mice will be bred with the intent of generating a cohort of 18 animals with confirmed tumor-load matching the following enrollment criteria:
    • Female mice are considered eligible for enrollment when ultrasound examination within 24 hours reveals 1 or 2 tumors, with the largest size being 6–11 mm and a combined tumor volume of 100–350 mm3.
    • Animals with three or more tumors per organ; cystic tumors; signs of intestinal, pancreatic, or gall bladder duct occlusions; or otherwise moribund/showing signs of dilapidation will not be included in the study.
  • Upon a single-dose injection, collect bleeds and biopsies of pre-specified tissue at five specified times (see Table A or B) after drug administration, processing three mice per each time point.
  • Include three vehicle-treated mice that are processed for blood and tissue collection at the last time point as drug-naïve controls. 
  • Provide cryopreserved plasma samples and tissues from PDAC tumor, lung, liver, and skeletal muscle for quantification of experimental compounds/metabolites.  
Table A
Group Treatment Dose Collection Time n
1 AE1 TBD, PO 1 hr 3
2 AE1 TBD, PO 2 hr 3
3 AE1 TBD, PO 4 hr 3
4 AE1 TBD, PO 12 hr 3
5 AE1 TBD, PO 24 hr 3
6 Vehicle TBD, PO 24 hr 3

 

Table B

Group Treatment Dose Collection Time n
1 AS1 TBD, IV 1 hr 3
2 AS1 TBD, IV 1 d 3
3 AS1 TBD, IV 3 d 3
4 AS1 TBD, IV 5 d 3
5 AS1 TBD, IV 7 d 3
6 Vehicle TBD, IV 7 d 3

Let's Talk

A portrait photo
Serguei Kozlov, Ph.D. Service Contact