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HIV/SIV Services
- Novel CD4+ T Cell Specific Immunohistochemistry Detection and Analysis Technical Service FNL Technical Service Number: ACVP-03
- Quantitative Assessment of Lymphoid Tissue Fibrosis Technical Service FNL Technical Service Number: ACVP-11
- Quantitative assessment of HIV or SIV viral DNA in laser capture microdissected immune cells Technical Service FNL Technical Service Number: ACVP-12
- Lineage-specific Next Gen Multiplex In Situ Hybridization for HIV or SIV RNA and DNA Technical Service FNL Technical Service Number: ACVP-14
Quantitative Assessment of Lymphoid Tissue Fibrosis
- Performs immunohistochemistry to determine the quantity of extracellular matrix deposits accumulated within secondary lymphoid tissues.
- Generates high-resolution digital images of each immunohistochemistry stained sample.
- Provides relevant data electronically, including a report with interpretations and conclusions.
The process:
- Collagen I, Collagen III, or Fibronectin immunohistochemistry is performed to ascertain the extent of extracellular matrix deposition (i.e., fibrosis) accumulated within secondary lymphoid tissues.
- To ensure consistency among assay batches, we utilize an automated immunohistochemistry system.
- High-resolution digital images of each IHC stained sample are generated using the Aperio ScanScope CS System.
- Regions of interest of a defined size are highlighted on each digital image using the Aperio ImageScope software regions tool.
- High-resolution images of regions of interest are generated using a custom software program created by the Imaging and Visualization Group that automatically extracts regions of interest from the digital slide annotation layer.
- Under the guidance of a subject matter expert, the core provides comprehensive quantitative image analysis using a custom series of unique actions in Photoshop with Fovea tools to determine the percent area in each high-resolution image that is occupied by fibrotic collagen.
Includes sufficient basic consultation with a subject matter expert.
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Claire Deleage, Ph.D.
Head, Tissue Analysis Core