The National Cancer Institute Mouse Repository is a resource for mouse cancer models, associated strains and mouse embryonic stem cells (mESCs) harboring inducible microRNA species supported by the Division of Cancer Biology and managed by the Frederick National Laboratory.
The repository is divided into two sections: Mouse model strains and mouse embryonic stem cell lines. You will be taken to two separate navigable databases for each. The contact is the same.
Mouse model strains are provided free of charge except for shipping costs through a Material Transfer Agreement (MTA). To request a mouse model strain, you will need to click the order form after selecting the specific strain you are interested in. Review the overall ordering process and MTA submission instructions.
Mouse embryonic stem cell lines are provided at a nominal fee of of $162.44 per ES cell line for processing and handling. Each cell line has a separate request form you will be asked to fill out from the available cell line listing.
Email us at MouseRepository@mail.nih.gov with any questions.
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Email us at MouseRepository@mail.nih.gov with any questions.
Mouse Models
The repository distributes more than 150 mouse models for human cancers. The models are archived as cryopreserved germplasm (embryos and/or sperm) and are available to the scientific community worldwide at no charge. The requestor is responsible for all shipping fees and must provide the liquid nitrogen dry shipper for the transport of the material.
- Cryoarchived and distributed as frozen germplasm (embryos and/or sperm)
- Governed by Material Transfer Agreements (MTA)
- No charge for the material, however, the recipient is responsible for shipping charges.
Mouse embryonic stem cell lines
The National Cancer Institute Division of Cancer Biology supports the generation of mouse embryonic stem cells (mESCs) harboring most known mouse microRNAs to address the role that microRNAs play in human cancer, their use as diagnostic tools, and their potential function as new targets for therapeutic intervention in the treatment of cancer.
The Repository offers 1,500 mESC lines for distribution at a nominal fee.
The miR ESC lines, when used to generate genetically engineered mice, will produce a tet-inducible, reversible system to investigate the role of each miRNA in vivo, providing a powerful tool for the study of miRNA biology.
Transgene expression for each ESC clone is controlled by either the tetracycline response element (TRE) or TRE-tight promoters, to accommodate a wide range of experimental designs. TRE-driven expression facilitates the study of microRNA in a variety of tissues, while TRE-tight promoter reduces leakiness and results in more restricted expression, suited for tissue-specificity, especially useful for study of miRNAs for which even the smallest level of expression may cause sterility or lethality.
- mES cell lines with mature microRNAs embedded in a miR30 precursor, designed to control the effect microRNA processing might have on overall expression
- mES cell lines with mature microRNA species in their endogenous context (referred to as: Primary-miRNAs)
- Parental ES cell line used to produce all the microRNA-expressing clones is KH2 (C57BL/6 x 129/Sv)
- All ES cell lines generated with this inducible system have been validated by flow cytometry for GFP expression
- Many have also been authenticated for miRNA expression as determined by RT-qPCR