Services

• Non-commercially available fluorochrome-labeled antibodies developed by the Cellular Immunity Core. 
  • SIV Env Clone 7D3 antibody, labeled with Alexa Fluor 647 
  • SIV Gag Clone 55-2F12 antibody, labeled with FITC 

• 30 ml to 30 L batch sizes
• Purify by sucrose density gradient ultracentrifugation
• Provide in infectious (non-treated) or chemically inactivated (using Aldrithiol-2)

• Derive from infectious stock or infectious molecular clone (preferred)
• Typically infect SUPT1 based cell line
• Derive stable, productively infected cell line
• May derive limiting dilution clone
• Produce and analyze purified virus preparation(s) to determine virus yield and biochemical phenotype.
• Test for contamination by adventitious agents: bacteria, mold, or mycoplasma.

• Imaging software  
• Machine learning, deep learning and AI 
• Bioinformatics workflows 
• Sequence annotation algorithms 
• Cryo EM pipeline 
• Power analysis 

Code Repositories

• Biomedical annotation and visualization resources 
• Genomic workflows 

Scientific Applications

Provides cryo-EM images from submitted specimens for cancer research. 

Submit an application

Researchers can request the following services from the Imaging Mass Cytometry Laboratory:  

• Building the metal-label antibody panel targeted to the project goals  
• Metal-labeling and validation of in-house conjugated and commercially available metal-label antibodies with known positive and negative control tissue slides.  
• Staining and acquisition of required number of tissue slides/tissue microarrays.  
• Sharing raw data from the instrument and assistance with high dimensional data analysis. 

If you are interested in requesting a service from the Imaging Mass Cytometry Laboratory, please contact Milind Pore or send an email to IMCFNLCR@nih.gov.