The RAS Reagents Research Team supplies nucleic acids, cell lines, and protein reagents for the RAS Initiative. We support the development of structural studies, drug screens, imaging experiments, and cell biology and biochemistry projects. Many of our reagents are available to the community.
RAS tools & resources
Our capabilities and specializations
Developing resources for RAS research
We have expressed and purified fully modified KRAS protein. This form of KRAS is required for signaling inside cells. Significant quantities have not been available until now.
We have provided insect cells and engineered baculovirus to researchers for producing their own fully modified KRAS protein.
Our team has been able to share 360 RAS pathway genes with the research community through Addgene. This is the only source for the entire set of RAS pathway genes. Importantly the isoform of each gene was chosen to match the forms most commonly found in human cancers.
The work of our group has led to the construction of >200 expression plasmids for protein purification, structural biology, cell line construction, and single molecule imaging studies. These are for use within the Frederick National Laboratory, but will be made available as their utility is demonstrated.
- Structural biology
- Single molecule imaging
- In vitro and in vivo drug screens
- Screens for vulnerabilities in cancer cell lines
- Gateway and Gateway Multisite cloning
- Libraries of promoters, fluorescent proteins, purification tags, labeling tags
- Bacterial, mammalian, insect, and viral vectors
- RAS-less mouse embryonic fibroblasts