Scientist I, RAS Mass Spectrometry (req2170)

Posted: 10/8/2021
Location: Frederick, MD
Employee Type: exempt full-time
Job ID: req2170

PROGRAM DESCRIPTION

The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.

JOB RESPONSIBILITIES: Scientist I (RAS Mass Spectrometry Group)

  • Support a range of collaborative NCI RAS Initiative projects encompassing global, targeted, and/or quantitative proteomics
  • Perform sample preparation and data acquisition/analysis for advanced top-down and peptide-based proteomic applications
  • Perform assay development for protein and PTM quantitation by peptide or proteoform reaction monitoring (PRM, PfRM)
  • Contribute to RAS proteoform assay expansion to encompass a broad range of cell, tissue, and cancer contexts
  • Assist with the daily operation and routine maintenance of three Orbitrap-based LC-MS platforms
  • Report, present, and publish research findings

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of a PhD degree from an accredited college or university according to the Council for Higher Education Accreditation (CHEA) in Chemistry, Biochemistry, Molecular Biology, or a related field or eight (8) years of related experience. Foreign degrees must be evaluated for U.S. equivalency
  • No experience necessary with doctoral degree
  • Knowledge or experience in sample preparation, data acquisition, and data analysis for quantitative proteomics and/or top-down proteomics (with accompanying publication record)
  • Knowledge of or experience in operating and maintaining Orbitrap-based instruments
  • Demonstrated ability to independently carry out experimental procedures, balance changing priorities, collaborate with a team, and perform troubleshooting to ensure timely project completion
  • Ability to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:

  • Prior experience in top-down proteomic applications (global and targeted), instrumentation, and data analysis
  • Prior experience in protein purification, protein biochemistry, and standard molecular biology workflows
  • Prior experience in native mass spectrometry or proteomic analysis of membrane protein complexes
  • Demonstrated ability to rapidly master new workflows, software, technology, and instrumentation

JOB HAZARDS

  • This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations