Research Technician (req2146)

Posted: 9/27/2021
Location: Frederick, MD
Employee Type: nonexempt full-time
Job ID: req2146

PROGRAM DESCRIPTION

The In Vivo Evaluation Group, BTB, DTP is establishing both in vitro and in vivo tumor lines from patient biopsies from DCTD sponsored clinical trials with the goal of developing models for NCI's Patient Derived Models Repository (PDMR). The PDMR is a repository of patient-derived models that include in vivo tumor fragments, in vitro tumor lines (2D and 3D), cancer associated fibroblasts, and DNA/RNA isolates.The goal of the PDMR is to distribute well validated and highly characterized material to researchers worldwide. A secondary mission is to evaluate the efficacy of approved oncology drugs in combination or as single agents in a subset of rare and recalcitrant PDX tumor models.

KEY ROLES/RESPONSIBILITIES

  • Aid in the daily maintenance, passaging and cryopreservation of the various 2D & 3D cultures grown in different medias
  • Establish organoid cultures from patient samples and from tumors isolated from mice implanted with human tumors
  • Prepare cultures for the various validation assays (flow cytometry, cell sorting, Q RT PCR, soft agar clonal assays, RNA/DNA extraction, the STR identity assay, proliferation assays, etc.)
  • Prepare all required medias, components and reagents used in the culture of organoid cultures
  • Participate in evaluating the effects of different basement membrane extracts, media additives, and different methodology used to establish and grow difficult 2D and 3D cell lines
  • Aid in other routine tasks as needed such as cell photography, PowerPoint presentations, tube/plate labeling, cell deliveries/pick-ups etc.
  • Maintain precise documentation of each 2D/3D cell line in Excel spreadsheets

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of a BS/BA degree from an accredited college or university (according to CHEA) (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
  • Ability to be flexible to meet changing needs
  • Organizational skills and database management skills
  • Ability to work in a fast paced environment
  • Ability to work underBiosafetyLevel-2conditions
  • Ability to work alone and as part of a team
  • Knowledge of Microsoft office programs (Word, Excel, PowerPoint)
  • Organizational skills and database management skills
  • Ability to maintain precise records
  • Good written and verbal communication skills and attention to detail
  • Ability to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:

  • Knowledge of sterile eukaryotic tissue culture techniques
  • Knowledge of organoid cell culture

JOB HAZARDS OR REQUIREMENTS

  • This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations