Research Associate II (req2229)

Posted: 10/28/2021
Location: Frederick, MD
Employee Type: exempt full-time
Job ID: req2229

PROGRAM DESCRIPTION

The Frederick National Laboratory for Cancer Research (FNL) is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by the prime contractor Leidos Biomedical Research, Inc. FNL is responsible for pursuing a unique mission in translational and applied biomedical research that is unsuitable for academia, the pharmaceutical industry, or other federal contractors. The selected candidate will join the Clinical PD Biomarkers support group located at the NCI-Frederick campus.

The Clinical PD Biomarkers support group is responsible for identification of biomarkers of response to new targeted agents entering clinical trials in the Developmental Therapeutics Program(DTP) of the National Cancer Institute. The scope of work includes assay design, development, validation, fitness for purpose testing, transfer of assays to clinical laboratories, and clinical specimen analysis when required. The program facilitates mechanistic and performance analysis of putative anti-cancer agents, diagnostic tests, and therapeutic interventions to clinical applications, including distinct molecular signatures for cancer, refined molecular assays, and state-of-the-art imaging techniques that are too high-risk for industry or academia to pursue. The program operates a number of advanced, specialized instrumentation for image analysis of tumor biopsies, circulating tumor cells, and activated T cells for measuring drug effects in patients enrolled in clinical trials.

KEY ROLES/RESPONSIBILITIES

  • Develop ELISA and multiplex assays of de novo pharmacodynamic biomarkers on the Luminex platform
  • Write “standard operating procedures” (SOPs)
  • Perform analytical validation of assays, processes assay data by appropriate analysis and statistical methodologies, and implements PD biomarker assays in preclinical and clinical studies
  • Independently performs complex assignments associated with all aspect of immunoassay development, validation, and implementation
  • Prepare and characterize reagents (antibodies, conjugates, calibrators, stabilizers, etc.) to support Luminex assays for clinical and preclinical testing. Characterize critical reagents using affinity purification, immunoblotting, and spectral analysis. Prepare quality control materials from xenograft tumor models, animal tissues and cancer cell line and qualifies them for different PD biomarker assays
  • Responsible for general laboratory upkeep to ensure compliance with relevant safety regulations and policies
  • Troubleshoot day-to-day activity on multiplex immunoassays to meet/ensure required performance
  • Perform SOP based cross-validation of PD biomarker assays
  • Handle general lab duties for Luminex Lab

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of Bachelor's degree from an accredited college/university according to the Council for Higher Education Accreditation (CHEA) or four (4) years relevant experience in lieu of degree. Foreign degrees must be evaluated for U.S. equivalency
  • In addition to the education requirement, a minimum of five (5) years of progressively responsible experience
  • 5yrs laboratory experience in techniques used in immunoassay development such as SDS-PAGE, Western blotting, antibody characterization, conjugation of proteins, protein purification, and cell culture
  • Proficient in writing laboratory procedures
  • Proficient in using Microsoft Office software (e.g., Outlook, Word, Excel, and PowerPoint)
  • Possession of higher education (MS) can be considered in lieu of experience
  • Should be able to independently design and conduct experiments, process quality control data, and process results into presentable scientific reports
  • Ability to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:

  • Experience in immunoassay development specifically on Luminex multiplex assay platform
  • High level knowledge of Microsoft Office programs with good overall computer skills
  • Minimum of two years of experience in multiplex assay development using the Luminex platform
  • Knowledge of de novo monoclonal antibody development

JOB HAZARDS OR REQUIREMENTS

  • This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations
  • This position is subject to working in or have the potential to exposure to high noise levels for extended periods of time requiring medical clearance
  • This position may require continuous or intermittent exposure to and exertion in environmental conditions such as working outdoors, temperature extremes, humidity extremes. etc. as part of the job