Research Associate I (req2112)

Posted: 9/2/2021
Location: Frederick, MD
Employee Type: exempt full-time
Job ID: req2112

PROGRAM DESCRIPTION

The Neutrophil Monitoring Laboratory, Clinical Services Program (Applied/ Developmental Directorate) provides clinical and basic laboratory support to Laboratory of Host Defenses and Laboratory of Clinical Infectious Diseases, NIAID.Data generated in the NML is used in the diagnosis and characterization of patients with various immunodeficiencies including chronic granulomatous disease (CGD), leukocyte adhesion deficiency (LAD), and hyper-immunoglobulinemia syndrome (JOB Syndrome).

KEY ROLES/RESPONSIBILITIES

  • Isolation of plasma and leukocyte subpopulations neutrophils from whole blood using different isolation protocols
  • Cryopreservation and archival of the isolated biological samples
  • Maintenance of databases and inventories of samples to be stored in the Central Repository
  • Performance of various commercial ELISAs for the analysis of neutrophil granule proteins and acute phase proteins
  • Isolation of nucleic acids (particularly DNA) from whole blood, saliva, or cells (PMN, DFP-treated PMN, PBMC,B cells, fibroblasts, or buccal swabs), using different methodologies (Oragene and Qiagene) and technologies (manual and automated)
  • Performance and analysis of complex CLIA-certified functional assays that include determination of superoxide generation by cytochrome c reduction, nitroblue tetrazolium staining, and PMN adherence to extracellular matrices
  • Recording temperatures of laboratory equipment in accordance to CLIA guidelines
  • Recording and maintaining reagent logs which describes the preparation and tracking of reagent usage in accordance to CLIA guidelines
  • Operation, maintenance, and quality control of biosensitive equipment including automated cell counters, spectrophotometer, cytometer, and plate readers
  • Work may involve the use of potentially hazardous chemical or biological materials or may be located in areas where such materials are used.Requires the use of personal protective equipment and adherence to strict safety protocols

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of Bachelor's degree from an accredited college/university according to the Council for Higher Education Accreditation (CHEA) or four (4) years relevant experience in lieu of degree. Foreign degrees must be evaluated for U.S. equivalency
  • In addition to the education requirement, a minimum of two (2) years of progressively responsible experience
  • Knowledge or experience in the isolation of cells from whole blood
  • Ability to perform complex assays and report results to supervisor or scientist, continually acquire new skills to enhance processes within a CLIA-certified [Clinical Laboratory Improvement Amendments of 1988] laboratory setting
  • Data triage and quality control evaluations
  • Maintain essential laboratory supplies and equipment
  • Operate and maintain sensitive biomedical equipment
  • Keep exact and detailed records of documents laboratory operations and data collection

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:

  • Ability to communicate effectively
  • Attention to detail

JOB HAZARDS

  • This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations