Protein Production Associate I/II (req2298)

Posted: 12/7/2021
Location: Frederick, MD
Employee Type: exempt full-time
Job ID: req2298


The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community and provides dedicated support to NCI's intramural Center for Cancer Research (CCR). The dedicated units provide expertise in electron microscopy, protein characterization, mass spectrometry, protein expression, optical microscopy, and genomics. These research efforts are an integral part of CCR at the Frederick National Laboratory for Cancer Research (FNLCR), where CRTP scientists work collaboratively with intramural NCI investigators to provide research technologies and expertise.


The ProteinExpression Laboratory (PEL) provides supportto various NCI and NIAID projects through generation and qualification ofprotein reagents for biochemistry, biophysics, structural biology, and drugscreening projects. The PEL creates expression constructs, expresses theencoded recombinant proteins in multiple expression systems, and purifies therecombinant proteins for use in downstream applications. The Protein Production Associate will:

  • Carry out experiments, under the review of a scientist, in the area of protein expression and purification
  • Perform microscale protein purification scouting
  • Perform large-scale protein purification using FPLC technology
  • Carry out extensive quality control on proteins to ensure high-quality reagent production
  • Maintain scientific equipment in good working order
  • Provide timely updates of project progress to supervisor and other staff in both informal and formal reports
  • Maintain detailed records of all laboratory processes and procedures for quality assurance purposes


To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of a Bachelor's degree in a related field, from an accredited college or university according to the Council for Higher Education Accreditation. Foreign degrees must be evaluated for U.S. equivalency
  • Research Associate I - In addition to the education requirement, a minimum of two (2) years of related biomedical experience
  • Research Associate II - In addition to the education requirement, a minimum of five (5) years of related biomedical experience
  • Applicants should have prior laboratory experience in protein purification
  • Ability to obtain andmaintain a security clearance


Candidates with these desired skills will be given preferential consideration:

  • Ability to handle multiple scientific projects with a high degree of accuracy
  • Experience with FPLC-based protein purification
  • Experience in microscale protein purification
  • Experience with affinity-based methods of recombinant protein purification, specifically IMAC
  • Experience with standard chromatographic modalities of IEX and SEC in the context of FPLC-based column chromatography


  • This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations