The Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Applied / Developmental Directorate, conducts Basic research, Translational Research, and Bioinformatics research.The goals of LHRI studies are to develop novel therapies for HIV(+) patients infected with multiple-class drug-resistant (MDR) HIV variants. This work is associated with NIAID clinical trials for HIV.LHRI has discovered the novel mutations associated with MDR or virus fitness in clinical samples and identified Interleukin-27 and Ku70 protein as a novel anti-HIV/HCV cytokine and a novel cytosolic DNA sensor inducing the innate immune responses, respectively.Recently, LHRI found potential novel targets in HIV to develop a novel therapy (J. Virology, 2021 doi: 10.1128/JVI.01323-21).
The Postdoctoral Fellows will be involved in the recently discovered HIV target study.
Performing multiple assays using qPCR, WB, Immune precipitation, confocal microscope, AMNIS (imaging flow cytometry), FPLC, cloning and constructing expression plasmids, knockdown or knock-in of gene of interests using (either siRNA or CRISPR), NGS
Publishing paper(s) and presenting in the international or domestic scientific conference(s)
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
Possession of a PhD degree from an accredited college or university according to the Council for Higher Education Accreditation (CHEA) or eight (8) years of related experience. Foreign degrees must be evaluated for U.S. equivalency
No work experience required with a Doctorate degree
Publication of the author paper(s)
Ability to obtain and maintain a security clearance
Candidates with these desired skills will be given preferential consideration:
General molecular biology techniques (RNA extraction, RT-PCR, genomic DNA isolation, construction of expression plasmids), tissue culture (primary human cells: T cells and macrophages, and cell lines ), transfection, western blotting, Immunol precipitation (pull-down) assay, FPLC or HPLC, Sanger DNA sequence. Docking modle analysis
Handling of HIV or other viruses
Tissue culture (cell line or primary human cells)
Knowledge of HIV virology
Statistical analysis skills
This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations