We perform multi-scale production (30ml to 30L per batch), chemical inactivation
treatment (if desired), purification, and associated quality control analyses of
retroviruses and related control reagents (microvesicles) that are used as vaccine
immunogens for animal studies and as key reagents for various in vitro studies.
Fluorescently labeled retrovirus and microvesicle preparations can also be prepared.
The BPC also prepares and distributes antigen capture immunoassay kits for the cost
effective measurement of HIV p24 antigen from in vitro samples.
We provide access to cutting edge sequence based analysis, including both single
genome amplification sequencing techniques and high throughput pyrosequencing technology,
coupled with genetic analyses. These approaches are useful for characterization
of virus stocks used for NHP studies, for evaluation of virus during transmission
and subsequent evolution, and for efficacy assessment of various intervention modalities.
The Retroviral Immunology Section studies cellular immune responses to retroviral
infection and is exploring the potential of adoptively transferred T lymphocytes
in controlling retroviral replication in vivo, using NHP models.
Due to the recent death of Dr. Ohlen, inquiries should be made to Dr. David Ott or Dr. Jeff Lifson.
The Retrovirology Pathogenesis Section studies various aspects of retroviral pathogenesis,
ranging from in vitro analyses of virus interactions with immune system cells to
in vivo studies of retroviral pathogenesis in NHP, including characterization of
basic pathogenetic mechanisms and evaluation of novel treatment and prevention modalities.
In vivo experiments include efforts to develop improved NHP models for vaccine evaluation
and testing of antiretroviral drug treatment and strategies for targeting residual
virus and latent reservoirs.
We maintain a cutting edge capability for detailed analysis of viral proteins and
host cell proteins associated with retroviruses, continuing to refine analytical
methods such as microscale HPLC fractionation and sensitive sequence and mass spectrometry
based analysis of viral and host cell proteins from highly purified preparations
The Viral Oncology Section conducts international molecular and serological epidemiologic
studies of infection with KSHV and other viruses, including international projects
with a local presence at the Medical Research Council's Uganda Virus Research Institute
in Entebbe, Uganda, along with basic studies of KSHV biology, with a recent emphasis
on the role of miRNA.
We consolidate the cellular immunology instrumentation and capabilities of the program
in support of ACVP investigators and collaborators, with advanced multiparameter
flow cytometry analysis and fluorescence activated cell sorting capabilities, including
the capacity for sorting of infectious specimens under appropriate biocontainment
We provide state-of-the art tissue analysis capabilities (i.e. Immunofluorescence,
immunohistochemistry, in situ hybridization, quantitative image analysis, multi-label studies, laser
capture microdissection) for directed studies and in support of intramural and extramural
investigator collaborative studies relevant to key questions in HIV/AIDS research,
with an emphasis on analysis of specimens from non-human primate (NHP) models.
Using quantitative PCR approaches for sequence detection and measurement, combined
with near quantitative methods for nucleic acid recovery from specimens, the QMDC
has become a recognized leader in the development, application and performance of
SIV viral load measurements.
The Retrovirus Cell Interaction Section examines critical aspects of how HIV-1 interacts
with its host by studying HIV-1 as well as SIV in the rhesus macaque animal model.
Important features of HIV-1 and SIV infection, replication, pathogenesis, and immunology
are investigated by the Section using a broad-based multidisciplinary experimental
approach. Areas of expertise include: retrovirology, molecular biology, cell biology,
and genetic engineering using retroviral and lentiviral vectors.
We serve as the "clinical laboratory" equivalent for specimens associated with studies
in NHP conducted by ACVP scientists and collaborators. The SSC receives, accessions,
processes, distributes, and stores blood and tissue derived specimens from macaques
under study, and maintains detailed databases on these specimens, including current
inventories of cryopreserved samples, and also performs standardized assays on these
HIV Molecular Monitoring Core (HMMC) was established in response to the increasing demand for specialized molecular
analyses of viral parameters in clinical studies in the AIDS research community. The key technologies resident
in the core will consist of an ultrasensitive viral load assay for HIV-1 (threshold limit of detection <1 HIV-1
RNA copy/mL of plasma), single genome sequencing methods, and analysis of genotypic markers of drug resistance.
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