Providing unparalleled technologies for detailed studies of molecular interactions.
Vast capabilities in surface plasmon resonance (SPR) spectroscopy and fluorescence
as well as complementary protein chemistry techniques for protein identification,
including mass spectrometry and classic Edman protein sequencing. The program has
state-of-the-art MALDI-TOF mass spectrometry with imaging and high mass detection
capabilities along with HPLC purification and quality control of protein and oligonuleotides.
Molecular Interactions: Characterizing interactions between proteins,
nucleic acids and small molecules
Protein Chemistry: Complete characterization of proteins of interest
Mass Spectrometry: PCL utilizes mass spectrometry technologies
including MALDI-TOF, MALDI-TOF/TOF and LC/MS/MS
Providing qualitative, quantitative, and structural analysis via mass spectrometry,
separations technology, and nuclear magnetic resonance. The program applies state-of-the-art
analytical technologies, primarily mass spectrometry, to advance the understanding
of cellular function at the proteomic and metabolomic levels. We also specialize
in the identification of individual proteins, characterization of entire protein
complexes, and global analysis to discover changes in the proteomes of different
Mass Spectrometry (MS): The program equipped with the latest in
mass spectrometry (MS) instrumentation for proteomic and etabolomics analysis. Areas
of expertise include protein complex characterization, global and target protein
quantitation, and identification of post-translational modifications.
Nuclear Magnetic Resonance (NMR): Program expertise in NMR experimentation
and analysis using the most current hardware and software. We provide characterization
of individual small molecules and metabolomic analysis for the comparative analysis
of complex mixtures of metabolites in clinical samples such as serum and urine.
Separations Technology (CE, HPLC, GC): The program has innovative
methods for separation and quantitation of analytes using gas chromatography (GC),
high-performance liquid chromatography (HPLC), and capillary electrophoresis (CE)
online with various detection methods such as MS and UV. They also specializes in
the development of highly accurate quantitative assays for specific molecules of
Providing the tools for cloning, protein expression, purification, and detection
technologies for the advancement of research. The program develops, improves and
delivers protein-centric capabilities.
The PEL Protein Pipeline:
The PEL has constructed hundreds of protein expression plasmids as well as clones that influence and report protein expression (shRNA, microRNA, reporter plasmids). The lab specializes in parallel small scale screening in bacteria, yeast, insect, and mammalian cells to optimize protein quality. An innovative “purify first” platform tests multiple expression modalities side by side for the most purifiable form of each target protein. Scale-up to tens of liters of culture and hundreds of milligrams of protein is routine.
Mammalian Viruses: PEL offers expertise in the production of custom recombinant lentivirus to study transgene or shRNA delivery for gene knockdowns in vitro and in vivo. Production services are complemented by offering virus concentration and titration services. Panels of ready-to-ship lentiviruses allow investigators to evaluate different promoters.
Molecular Assays: PEL provides cell line quality control and authentication including mycoplasma testing, screens for a subset of human viral pathogens and short tandem repeat (STR) cell line authentication.
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