Molecular Technique

Multiplatform Next generation sequencing

FNLCR laboratories are equipped with the most sophisticated next generation sequencing technology including Illumina, Life Technologies, Roche 454 and Pacific Biosciences instruments. FNLCR staff are highly trained in library construction techniques and can handle the most challenging sequencing projects. FNLCR is equipped with the computational storage, processors and bioinformatics staff to analyze and deliver results for any sequencing project with particular expertise n combinig seqeunce results from multiple platforms.

Epigenetic modifications

Modifications in DNA and chromatin can have a profound effect within cellular systems. The FNLCR has strong capabilities in the detection of epigentic modifications including ChIP-Seq on the Illumina platforms, Single Molecule detection of base modifications on the PacBio sequencer and targeed quantitative measurements using the Qiagen Pyromark system

DNA Structure

Alterations in DNA structure as a result of DNA rearrangements, Viral integration or transgenic manipulation can have a profound effect on cellular phenotype. The FNLCR has capabilities to evalaute structural alterations using both Array and Next generation sequencing approaches.

Gene Expression

The FNLCR has outstanding facilities for measuring gene expression differences that may be used in both basic and translational science. Capabilities include a full range of Affymetrix single cartridge and high throughput arrays, RNA-Seq protocols,Agilent custom arrays, and microfluidic real time PCR. Scientists within the laboratory are avaialble to consult on project design and to participate in the interpretation of experimental results.

Single cell genomics

Technical advances now allow measurements of DNA and RNA on single tumor cells. The FNLCR has capablites to make single cell gene expression measurements and will be establishing single cell genomic sequencing in the latter half of 2013