The Center for Advanced Preclinical Research (CAPR), directed by the NCI Center
for Cancer Research, portfolio of genetically engineered mice (GEMs) includes models
for non-small cell lung cancer, serous epithelium ovarian carcinoma, highgrade astrocytoma/glioblastoma,
melanoma, pancreatic adenocarcinoma and prostate carcinoma. Tumors in these models
are evaluated by various in vivo imaging techniques and by histopathologic and molecular
analyses. For several models, orthotopic transplantation versions are available
for accelerated expansion of experimental cohorts.
We use highly specific, highly sensitive tools to detect protein expression in multitudes
of tissue from several species. Using antibodies, minute concentrations of specific
proteins can be visualized within the confines of the microscopic/ macroscopic environment
from which the protein naturally resides. Antibody mediated staining can be used
to confirm protein expression, detect protein co-localization, isolate cell populations,
elucidate tumor locality, functionality, and/or type, and otherwise present a “snap-shot”
of the tissue/cells for a specific target of interest. With around 20 years of experience,
the PHL Immunohistochemistry department provides reliable and consistent results while continually
developing novel techniques to meet investigator needs.
We employ several culture methods to identify pathogenic organisms appropriate to
the animal species. The laboratory has the capabilities to provide more sophisticated
culture techniques for clinical cases, including anaerobic and special gas environments,
phase microscopy, and serological typing.
We have developed and validated PCR-based assays for a number of murine viral and
bacterial pathogens. Currently, the PCR-based assays are utilized in conjunction
with traditionally based assays. This allows the AHDL to utilize two independent
approaches to health monitoring. New assays for various rodent pathogens are constantly
being developed and enhanced.
Our pathologists function as scientific collaborators assisting in animal study
design, species selection, histopathology and immunohistopathology evaluation, photomicrography,
report and manuscript preparation.
With our complete qualitative and quantitative analysis of samples ranging from
whole mice to specific anatomical microenvironments we are able to provide comprehensive
reports with raw and processed data presented to the researcher along with all the
images used to conduct the analysis. Whether looking at a single population of cells,
comparing multiple populations of cells, examining organs from control animals and
transgenic animals, or demonstrating percent of metastasis in a single organ, there
is nearly a limitless amount of information that can be obtained. A library of routine
analysis techniques is maintained in addition to working closely with researchers
to develop novel techniques yielding the most robust and useful information for
The technical preparation of tissue samples is performed to the highest standards
through the preparation of microscope slides following highly specific LCM protocols
that have been developed to maximize both the quality and quantity of nucleic acids
in the sample. The laboratory has successfully isolated nucleic acids not only from
newly acquired material, but also archived tissues and blocks. A team of veterinary
pathologists is on hand to collaborate with researchers, and supervise the selection
of target cell populations.
The Molecular Pathology Group (MPG) is part of the Laboratory Aniaml Sciences Program (LASP), at the FNLCR.
We are actively working to expand high-quality, pathology-based research application
support available to investigators and their collaborators. Its primary role
is to extend the capabilities of the FNLCR Pathology and
Histology Laboratory (PHL) core group by coordinating and performing advanced, tissue-based
molecular imaging studies at the request of investigators. Our goal is to be a flexible extension to your laboratory.
We have the capability to detect viral and mycoplasmal antibody utilizing traditional
and modern techniques, including HI, ELISA, and IFA tests. The AHDL utilizes an
independent commercial laboratory for confirmation of unusual test results.
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